BACKGROUND/AIMS

The aim of the present study was to compare Quick Test (QT) in the diagnosis of subclinical mastitis related with human health risk with California Mastitis Test (CMT) and to determine the compatibility of QT with Somatic Cell Count (SCC) using reference data from Fossomatic.

MATERIAL and METHODS

The study was performed using milk samples collected from 160 individual udder quarters of 40 Holstein cows at three different farms. Milk samples were initially checked by CMT and QT. Then, the samples were sent for SCC analyses.

RESULTS

The SCC results (0-100,000 cell/mL) of 101 (101/160) samples belonging to ≤100 standards of QT category were found to be compatible in the ratio of 60.39%. When QT standards of ≤100 and 250 were compared with the SCC results, the negative subclinical mastitis values (0-250,000 cell/mL) were compatible in the ratio of 84%. When CMT was compared with SCC, the negative subclinical mastitis values of CMT were found to be compatible with the SCC results (0-250,000 cell/mL) in the ratio of 85.9%. For the determination of all cases with subclinical mastitis, SCC data were found to be compatible with QT and CMT in the ratios of 97.6% and 63.4%, respectively. When QT and SCC were compared with each other, the specificity, sensitivity, and false ratio of QT were detected as 84.0%, 97.5%, and 16.7%, respectively.

CONCLUSION

Quick Test is a supporting method to other tests or alternative method to CMT in the diagnosis of subclinical mastitis related with human health risk.

Keywords: California mastitis test, quick test, somatic cell count, subclinical mastitis