ABSTRACT

Enterecoccus faecalis is the most dominant and most isolated resistant species from infected/failed permanent teeth undergoing root canal therapy or painful primary teeth with periapical radiolucency. The main purpose of the present research was to compare the antimicrobial effectiveness of essential oil of Origanum onites and diode laser irradiation against E. faecalis removal from primary root canals.

A total of 60 retained human primary incisors were selected randomly and divided into six experimental groups as follows: Group 1: negative control; Group 2: positive control; Group 3: sodium hypochlorite (NaOCl); Group 4: diode laser; Group 5: Origanum onites (OO) oil; Group 6: diode laser þ OO oil. The gas chromatography–mass spectrometry (GC–MS) and GC analyses of OO oil were carried out with an Agilent 5977B GC-MSD and Agilent 7890B GC systems, respectively. A 2-W diode laser was used in a continuous action mode with a wavelength of 980nm for 20 seconds. NaOCl and OO oil were applied continuously for 5 minutes to each root canal. Multiple comparisons and the significances between experimental groups were statistically analyzed using Tukey’s multiple comparisons test. The level of significance was accepted at .05 for the entire statistical analysis (P ¼ .05).

The major reductions were observed in the groups of NaOCl (98.3%) and diode laser þ Origanum onites oil combination (92.5%).

Combined therapy of diode laser irradiation following Origanum onites oil application in primary root canal disinfection may be used as an ideal effective chemo-mechanical alternative to NaOCl irrigation.