ABSTRACT

The aim of this study was to determine the early signal of methicillin-resistant Staphylococcus aureus (MRSA) growth in blood culture bottles.

The MRSA infections should be determined specially for the hospitalized patients to decrease the rates of mortality and morbidity. 19 MRSA isolates were included in the study. Strains were identified using the Phoenix 100 system (Becton-Dickinson Diagnostic Systems, Sparks, MD, USA). Three pre incubation grades were analysis: group I: 4°C, group II: 25°C, and group III: 37°C. Blood culture bottles were pre-incubated for 24 hours prior to the inoculation with MRSA. Contaminated bottles were incubated in the Bactec 3D Alert (Becton- Dickinson Diagnostic Systems, Sparks, MD, USA).

As indicated by the three different pre-incubation groups, the initial signals were detected at 37°C. The MRSA were grown for 12 hours in 37°C blood culture bottles.

Blood culture bottles which thermostatized at 37°C during 24 hours could give the early signal to determine the MRSA.