Cyprus Journal of Medical Sciences
Original Article

Evaluation of the Apoptotic Effects of the Humic Acid Treatment on Chronic Myeloid Leukemia Cell Line K562


Department of Biophysics, Marmara University Faculty of Medicine, İstanbul, Turkey


Department of Medical Biology, Near East University Vocational School of Health Sciences, Nicosia, Cyprus


Department of Biophysics, Near East University Faculty of Medicine, Nicosia, Cyprus

Cyprus J Med Sci 2021; 6: 157-161
DOI: 10.5152/cjms.2021.3044
Read: 18 Downloads: 20 Published: 14 July 2021

Humic acid (HA) formed by the decomposition of organic matter is a high-molecular-weight polymer. Humic-derived substances are potential drugs for human health, and their role in the prevention and treatment of diseases, like diabetes, antiviral, UV-B protective effect, cancer, and cardiovascular disease, is emphasized in various studies. It has antiulcerogenic, antitumor. Yang et al. demonstrated that HA exerts an antiproliferative effect and growth inhibition on HL-60 cells through the induction of apoptosis. A study showed that the antiulcer effect of Shilajit (HAs extracted from Shilajit) samples may be due to antimicrobial, anti-inflammatory, antianxiety, antioxidant, healing, and regenerative effects. The present study aimed to investigate the effects of HA on cultures of the chronic myeloid leukemia cell line in vitro.

We determined the apoptotic effect of HA in K562 cells by using various molecular methods. For cell viability, HA was tested in vitro against K562 cell lines using the MTT colorimetric process. Bax, bcl-2, casp-3, and casp-9 expressions were quantified by Western blot. Apoptosis analysis has been made with the Annexin-V/PI method.

The IC50 values of HA were determined 100 mg mL1 for the K562 cell line. HA has been shown to inhibit cell proliferation. As the bcl-2/bax ratio decreased and increasing the levels of caspase-3 and caspase-9, the cells underwent apoptosis.

As a result, HA showed the anticancer effect on leukemia cells. In the future, HA can be used as a chemopreventive agent in leukemia treatments.

Cite this article as: Tiber PM, Balcı-Okcanog˘lu T, Karadeniz M, Aykac¸ A. Evaluation of the Apoptotic Effects of the Humic Acid Treatment on Chronic Myeloid Leukemia Cell Line K562. Cyprus J Med Sci 2021; 6(2): 157-161.

ISSN 2149-7893 EISSN 2536-507X