Cyprus Journal of Medical Sciences
Original Article
Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia

Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia

1.

Department of Medical Biology, Near East University Vocational School of Health Services, Nicosia, Cyprus

2.

Department of Medical Biology, Ege University School of Medicine, İzmir, Turkey

3.

Department of Hematology, Ege University School of Medicine, İzmir, Turkey

Cyprus J Med Sci 2020; 5: 153-156
DOI: 10.5152/cjms.2020.1542
Keywords : Apoptosis, Genistein, HL-60, hTERT
Read: 52 Downloads: 12 Published: 29 June 2020

BACKGROUND/AIMS: The purpose of this study was to research the effects of genistein on telomerase activity and apoptosis in an acute promyelocytic leukemia cell line (HL-60).

MATERIAL and METHODS: In HL-60 cells, the cytotoxic effect of commercially available genistein was evaluated by the XTT method. The XTT method is a Cell Proliferation Assay. The Annexin V-EGFP method was used to determine apoptosis. The human telomerase reverse transcriptase (hTERT) is a marker of telomerase activity. hTERT gene expression analysis was performed by LightCycler real-time RT-PCR.

RESULTS: In HL-60 cells, the IC50 of genistein was found to be 50 µM at 72 hours. It was observed that the induction of apoptosis was 4.25-fold higher compared to the genistein untreated cells used as the control group. Compared to the control group, hTERT activity was found to decrease by 5.16, 3.81 and 5.04-fold at 24, 48 and 72 hours, respectively.

CONCLUSION: Induced apoptosis of HL-60 cells by the reduction of human telomerase reverse transcriptase activity may be a beneficial parameter in leukemia patients.

Cite this article as: Okcanoğlu TB, Avcı ÇB, Yılmaz Süslüer S, Kayabaşı Ç, Saydam G, Gündüz C. Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia. Cyprus J Med Sci 2020; 5(2): 153-6.

Files
ISSN2149-7893 EISSN 2536-507X