Cyprus Journal of Medical Sciences
Original Article

Protective Effect of Nigella Sativa Oil Against Indomethacin-Related Small Intestine and Gastric Mucosal Damage in Rats


Department of General Surgery, Haydarpasa Numune Research Hospital, İstanbul, Turkey


Department of Biophysics, Near East University School of Medicine, Mersin, Turkey


Department of Biochemistry, Vocational School of Health Related Professions, Marmara University, İstanbul, Turkey


Department of Histology and Embriology, Cyprus Dunya IVF Center, Mersin, Turkey


Department of Histology and Embryology, Marmara University School of Medicine, İstanbul, Turkey


Department of Pharmacology, Near East University School of Dentistry, Mersin, Turkey

Cyprus J Med Sci 2021; 6: 8-12
DOI: 10.5152/cjms.2021.1045
Read: 59 Downloads: 43 Published: 22 April 2021

The aim of this study was to investigate the effects of Nigella sativa (NS) oil form on reducing the damage caused by indomethacin in the stomach and duodenum of rats owing to their antioxidant and anti-inflammatory properties. 

The rats were divided into 4 groups: group 1, saline-treated control group; group 2, NS-treated control group; group 3, saline-treated ulcer group and ulcers caused by indomethacin (30 mg/kg) and administration of physiological serum; group 4, NS-treated ulcer group, which is the group receiving NS oil after administration of indomethacin. At the end of the study, blood samples collected from animals were examined for tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), and glutathione (GSH), malondialdehyde (MDA) levels and myeloperoxidase (MPO),and Na+/K+-ATPase activities in gastric and intestinal tissue samples. 

Levels of TNF-α and IL-1β in serum and MDA and MPO values in tissue were found to be higher in the saline-treated ulcer group than in the saline-treated control group. In addition, tissue GSH and Na+/K+-ATPase levels were found to be lower. These values were found to be reversed when comparing NS-treated ulcer group to saline-treated ulcer group. Histopathological findings showed epithelial regeneration and improvement instead of dense tissue damage. 

The strong antioxidant and anti-inflammatory effects of NS against potential small intestine and gastric damage were shown using an experimental indomethacin-induced ulcer model in rats. Hence, our study suggests that NS used together with indomethacin can prevent gastrointestinal damage; thus, this agent can create a new clinical therapeutic principle.

Cite this article as: Günay E, Özkan E, Abuoğlu HH, Aykaç A, Velioğlu Öğünç A, Karanlık B, Çetinel Ş, Şehirli AÖ. Protective Effect of Nigella Sativa Oil Against Indomethacin-Related Small Intestine and Gastric Mucosal Damage in Rats. Cyprus J Med Sci 2020; 6(1): 8-12.

ISSN 2149-7893 EISSN 2536-507X