Cyprus Journal of Medical Sciences
Original Article

The Suitability of Silk Fibroin Based Biofilms for Cartilage Tissue Engineering

1.

Department of Biomedical Engineering, Near East University Facult of Engineering, Mersin, Turkey

2.

Tissue Engineering and Biomaterials Research Center, Center of Excellence, Near East University, Mersin, Turkey

3.

Department of Medical Genetics, Near East University Faculty of Medicine, Mersin, Turkey

4.

DESAM Institute, Near East University, Mersin, Turkey

5.

SUNUM Nanotechnology Research Center, Sabancı University, İstanbul, Turkey

Cyprus J Med Sci 2021; 6: 124-128
DOI: 10.5152/cjms.2021.1914
Read: 252 Downloads: 86 Published: 14 July 2021

BACKGROUND/AIMS
Silkworms and spiders produce silk fibroin (SF). SF protein has unique characteristics, which includes mechanical properties, biodegradation, biocompatibility, and the ability to support the differentiation of stem cells along the osteogenic lineage. These characteristics makes SF a favorable biomaterial for cartilage tissue engineering. The aim of this study was to design a SF biofilm and then to test the biocompatibility and cytotoxicity of the designed SF biofilm.

MATERIAL and METHODS
Characterization was executed by scanning electron microscopy and Fourier transform infrared spectrophotometer analysis spectrophotometry. Normal human articular chondrocytes were seeded on biofilm and cultured up to 2 weeks (5% CO2, 95% air and 37C) under the standard culture conditions. Phase contrast microscopy and cell proliferation assay (3-(4, 5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay) was applied for evaluation of cell attachment and cell growth.

RESULTS
The viability of cellswas linearly correlated with optical density, and chondrocyte viability in the SF filmwas found to be significantly higher.

CONCLUSION
These results indicated that SF film supports cell proliferation without side effects and the SF film is a potential material as a cartilage tissue engineering matrix.

Cite this article as: Nwekwo CW, Kalkan R, Adali T. The Suitability of Silk Fibroin Based Biofilms for Cartilage Tissue Engineering. Cyprus J Med Sci 2021; 6(2): 124-128.

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ISSN 2149-7893 EISSN 2536-507X