Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia
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Original Article
P: 153-156
June 2020

Genistein-Induced Apoptosis Affects Human Telomerase Reverse Transcriptase Activity in Acute Promyelocytic Leukemia

Cyprus J Med Sci 2020;5(2):153-156
1. Department of Medical Biology, Near East University Vocational School of Health Services, Nicosia, Cyprus
2. Department of Medical Biology, Ege University School of Medicine, İzmir, Turkey
3. Department of Hematology, Ege University School of Medicine, İzmir, Turkey
No information available.
No information available
Received Date: 22.11.2019
Accepted Date: 04.03.2020
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ABSTRACT

BACKGROUND/AIMS

The purpose of this study was to research the effects of genistein on telomerase activity and apoptosis in an acute promyelocytic leukemia cell line (HL-60).

MATERIAL and METHODS

In HL-60 cells, the cytotoxic effect of commercially available genistein was evaluated by the XTT method. The XTT method is a Cell Proliferation Assay. The Annexin V-EGFP method was used to determine apoptosis. The human telomerase reverse transcriptase (hTERT) is a marker of telomerase activity. hTERT gene expression analysis was performed by LightCycler real-time RT-PCR.

RESULTS

In HL-60 cells, the IC50 of genistein was found to be 50 μM at 72 hours. It was observed that the induction of apoptosis was 4.25-fold higher compared to the genistein untreated cells used as the control group. Compared to the control group, hTERT activity was found to decrease by 5.16, 3.81 and 5.04-fold at 24, 48 and 72 hours, respectively.

CONCLUSION

Induced apoptosis of HL-60 cells by the reduction of human telomerase reverse transcriptase activity may be a beneficial parameter in leukemia patients.